2 edition of Some aspects of the host involvement in cowpea mosaic virus replication. found in the catalog.
Some aspects of the host involvement in cowpea mosaic virus replication.
Amarilis Paula Alberti de Varennes e. Mendonca
Thesis (Ph.D.), School of Biological Sciences, University of East Anglia, 1985.
Cowpea mosaic virus (CPMV) or Cauliﬂower mosaic virus (CaMV), are involved in dramatically restructuring the PD by apparently removing the desmotubule, the appressed endoplasmic reticulum (ER) within PD, leading to an expan-sion of the pore to allow for movement of virions with diam-eters up to 50 nm. These viruses are thought to convert. Soybean mosaic virus (SMV) is a member of the potyvirus group (Hill, ).The virus particle is a long, flexuous rod containing a single strand of RNA. Over 30 species of aphid including Aphis glycines, the soybean aphid, transmit the virus incidences of SMV correlate with flights of the soybean aphid (Burrows et al., ).With any of the aphid vectors, the stylet transmits.
As viruses are obligate intracellular pathogens they cannot replicate without the machinery and metabolism of a host cell. Although the replicative life cycle of viruses differs greatly between species and category of virus, there are six basic stages that are essential for viral replication. 1. Attachment: Viral proteins on the capsid or phospholipid envelope interact with. Cowpea mosaic virus (CPMV) was the first plant virus to be developed as an epitope presentation system (Usha et al., ; Porta et al., , ).CPMV is a bipartite RNA virus (Fig. ), with particles containing 60 copies each of a large (L; 37 kDa) and a small (S; 23 kDa) CP arranged with icosahedral symmetry (Fig. ).The virus was an attractive candidate for development as an.
If a host lacks the receptor for a virus, or if the host cell lacks some component necessary for the replication of a virus, the host will inherently be resistant to that virus. For example, mice lack the receptors for polio viruses and thus are resistant to polio virus. Similarly, humans are inherently resistant to plant and many animal viruses. Start studying Chapter 16 Biology. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The stage of viral replication in which the host cell produces viral nucleic acids and proteins is grafting, seed, pollen, and being carried on the mouth parts of chewing insects. Tobacco mosaic virus is most commonly.
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Some aspects of the host involvement in cowpea mosaic virus replication Author: Mendonca, A. Taxonomy: Cowpea mosaic virus (CPMV) is the type member of the Comoviridae and bears a strong resemblance to animal picornaviruses, both in gene organization and in the amino acid sequence of replication proteins.
Little systematic work has been done to compare isolates of the virus from different parts of the world. Physical properties: Purified preparations of virus contain three centrifugal Cited by: The host-encoded kDa monomeric RNA-dependent RNA polymerase, which is known to be stimulated in CPMV-infected cowpea leaves, did not copurify with the virus-specific RNA polymerase complex.
Our results dispute the hypothesis that plant viral RNA replication may be mediated by the RNA-dependent RNA polymerase of uninfected by: Cowpea mosaic virus causes one of the most commonly reported virus diseases of cowpea (Vigna unguiculata), in which it produces chlorotic spots with diffuse borders in inoculated primary leaves.
Participation of the Cowpea mosaic virus protease in eliciting extreme resistance. Replication of cowpea mosaic virus in protoplasts isolated from immune lines of cowpeas impede the host. Eggen R, Verver J, Wellink J, Pley K, Van Kämmen A, Goldbach R () Analysis of sequences involved in cowpea mosaic virus RNA replication using site specific mutants.
Virology – PubMed CrossRef Google Scholar. The type species, cowpea mosaic virus (CPMV), is the most thoroughly studied with respect to genome structure, replication, translation strategy, and virus-host interactions. Coalescence of the Sites of Cowpea Mosaic Virus RNA Replication into a Cytopathic Structure Article (PDF Available) in Journal of Virology 76(12) July with 87 Reads.
VIROLOGY() Independent Replication of Cowpea Mosaic Virus Bottom Component RNA: In Vivo Instability of the Viral RNAs AMARILIS DE VARENN AND ANDREW J.
MAULE1 John Inn Institute, Colney Lane, Norwich NRi. 7UH, United Kingdom Received March 7, ; accepted April 2, We compared the accumulation of cowpea mosaic virus (CPMV) RNAs after inoculation of cowpea.
In addition to the above mentioned roles that the host secretory pathway may play in the transport of virus replication complexes and viral and host proteins required for efficient spread of viruses, it is worth noting that the TGB2 protein of potato mot top virus (PMTV), which is located to both the ER and PD and is probably involved in the modification of PD for intercellular virus movement (Cowan et.
Replication of a DNA virus is shown in (1); replication of an RNA virus is displayed in (2). For the release of new viral particles, any of a number of processes may occur.
For example, the host cell may be “biochemically exhausted,” and it may disintegrate, thereby releasing the virions. Cowpea mosaic virus (CPMV) replication induces an extensive proliferation of endoplasmic reticulum (ER) membranes, leading to the formation of small membranous vesicles where viral RNA replication takes place.
Thesis. Some aspects of the host involvement in cowpea mosaic virus replication () Amarilis Paula Alberti de Varennes e Mendonça (born 1 November ) is a Portuguese academic who is currently a Professor at the University of Lisbon and President of the Instituto Superior de Agronomia.
She graduated from the Technical University of Lisbon in and completed her PhD entitled "Some aspects of the host involvement in cowpea mosaic virus replication. Viruses, thus, utilize many host factors for efficient viral replication in host cell.
Virus-host interactions are crucial determinations of host range, replication, and pathology. A fraction which contained the membrane-bound cowpea mosaic virus RNA replicase was isolated from cowpea mosaic virus-infected cowpea leaves. The replicase activity appeared on day 1 after inoculation, then increased to reach a maximal on day 4.
The increase in enzyme activity preceded the most-rapid virus multiplication. Cowpea mosaic virus is one of the best studied RNA viruses that employ polyprotein processing as a translation strategy. Still, many aspects of the viral infection process have remained obscure so far, e.g.
the RNA replication process, translocation through plant tissue, and various aspects of virus-host. ment to specific receptors host cell with host cell membrane nucleic acid into the host cell c info on nucleic acid provides the instructions for the host cell to start to produce viral components (hijacking) virus particles made therefore new viruses are made (sometimes particles take part of the cell).
Adsorption - virus binds to the host cell. Penetration - virus injects its genome into host cell. Viral Genome Replication - viral genome replicates using the host's cellular machinery.
Assembly - viral components and enzymes are produced and begin to assemble. Maturation - viral components assemble and viruses fully develop.
susceptible to Tobacco leaf curl virus, which causes a yellowing disease. In Western Europe in the period from about tomany paintings and drawings were made of tulips that demonstrate flower symptoms of virus disease.
These are recorded in the Herbals of the time and some of the ear-liest in the still-life paintings of artists such as. Attachment of animal viruses to host cells is random and nonspecific.
Animal viral DNA that is integrated into the host chromosome is called a provirus. Enveloped viruses are released from the cell by budding. Uncoating can occur due to host cell lysosome action.
Penetration of enveloped viruses can occur by a process called fusion. 2 Viral Replication: Basic Concepts • Replication cycle produces-Functional RNA’s and proteins-Genomic RNA or DNA and structural proteins• ’s-1,’s new particles produced by each cycle-Referred to as burst size-Many are defective-End of ‘eclipse’ phase• Replication may be cytolytic or non-cytolytic Steps in Viral Replication: Attachment.Viral replication is the term used indicate the formation of biological viruses during the infection process in the target host cells.
Viruses must first penetrate and enter the cell before viral replication can occur. From the perspective of the virus, the purpose of viral replication is .Besides translation, some host translation factors, including eukaryotic elongation factors (eEFs), the Lsm1–7 complex, and DEAD-box helicases, are integrated into the VRC and utilized for robust viral RNA replication.
At least in some plant–virus interactions, plants can recognize virus infections and induce translation repression to.